UFM1 Antibody / Ubiquitin-fold modifier 1 Antibody (FY13526)

UFM1 Antibody by IF in human thyroid carcinoma tissue. Immunofluorescence staining of a formalin-fixed, paraffin-embedded human thyroid carcinoma section using Ubiquitin-fold modifier 1 Antibody reveals intense red fluorescence within epithelial cell groups surrounding glandular lumens. Blue DAPI nuclear counterstaining delineates the cellular architecture, while the red signal is predominantly extranuclear and varies among cells. Sections underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, and incubation with primary antibody at 5 ug/mL overnight at 4oC. A Cy3-conjugated secondary antibody was used for detection. The image demonstrates UFM1-associated staining within the examined thyroid tumor tissue.

UFM1 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human thyroid carcinoma using Ubiquitin-fold modifier 1 Antibody demonstrates brown immunoreactivity in gland-forming epithelial cells. Staining is evident in cytoplasmic regions around several glandular lumens, with variation in intensity between adjacent cells. Blue hematoxylin counterstaining outlines the nuclei and surrounding tissue. Antigen retrieval was performed in EDTA buffer, pH 8.0, followed by overnight incubation with primary antibody at 2 ug/mL at 4oC and HRP/DAB detection. The distinct glandular architecture allows localization of UFM1-associated signal within this tissue section.

UFM1 Antibody by WB in human cell lines and rodent liver. Western blot analysis using Ubiquitin-fold modifier 1 Antibody detects a band near 10 kDa in human 293T (lane 1), HepG2 (lane 2), SH-SY5Y (lane 3), and U251 (lane 4) cell lysates, rat liver (lane 5), and mouse liver (lane 6). The observed migration agrees with the approximately 9-10 kDa predicted mass of unconjugated UFM1. Band intensity varies modestly across the samples, with additional faint higher-molecular-weight signals in some lanes. Reduced lysates containing 30 ug protein per lane were resolved on 12% SDS-PAGE; primary antibody was applied at 0.5 ug/mL overnight at 4oC before chemiluminescent detection.

UFM1 Antibody by IHC in human pancreatic carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreatic carcinoma using Ubiquitin-fold modifier 1 Antibody shows patchy brown staining in epithelial tumor cells, predominantly around the cytoplasm and cell borders. Areas of connective tissue and pale stroma exhibit comparatively little reaction product. Hematoxylin counterstaining provides blue nuclear contrast. Sections underwent heat-induced antigen retrieval in EDTA buffer, pH 8.0, followed by overnight incubation with primary antibody at 2 ug/mL at 4oC and HRP/DAB visualization. The heterogeneous distribution illustrates UFM1-associated immunoreactivity in the examined pancreatic tumor section.

UFM1 Antibody by FACS in mouse RM-1 cells. Intracellular flow cytometry using Ubiquitin-fold modifier 1 Antibody shows a blue fluorescence histogram shifted to the right of the green isotype-control distribution and red unstained control in RM-1 cells. Cells were fixed with 4% paraformaldehyde, permeabilized, and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. A fluorescent anti-rabbit secondary antibody was used for detection. The separation of the antibody-stained population from both controls indicates detectable intracellular UFM1-associated immunoreactivity in these mouse cells.

UFM1 Antibody by FACS in human HepG2 cells. Intracellular flow cytometry using Ubiquitin-fold modifier 1 Antibody demonstrates a rightward shift of the blue antibody-stained population relative to the green isotype-control and red blank-control histograms in HepG2 cells. Cells were fixed, permeabilized, blocked with goat serum, and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. Fluorescence was measured following incubation with a labeled anti-rabbit secondary antibody. The separated distributions demonstrate UFM1-associated staining above the controls in this human hepatocellular cell line.

UFM1 Antibody by FACS in rat RH35 cells. Intracellular flow cytometry using Ubiquitin-fold modifier 1 Antibody reveals a blue fluorescence peak to the right of the green isotype-control and red blank-control peaks in RH35 cells. Following fixation and permeabilization, cells were incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC and then stained with a fluorescent anti-rabbit secondary antibody. The antibody-positive histogram is distinguishable from the controls, supporting detection of intracellular UFM1-associated antigen in the examined rat cell population.