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Home >> Antibodies >> UDP-glucose 4-epimerase Antibody / GALE

UDP-glucose 4-epimerase Antibody / GALE (F54898)

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  Catalog No Formulation Size Price (USD)  
Image F54898-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54898-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human skin tissue with UDP-glucose 4-epimerase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human skin tissue with UDP-glucose 4-epimerase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human WiDr cells with UDP-glucose 4-epimerase antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human A375 cell lysate with UDP-glucose 4-epimerase antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human HeLa cells with UDP-glucose 4-epimerase antibody; Blue=isotype control, Green= UDP-glucose 4-epimerase antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q14376
Localization Cytoplasmic
Applications Immunofluorescence : 1:10-1:50
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500-1:1000
Limitations This UDP-glucose 4-epimerase antibody is available for research use only.
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Description

UDP-galactose-4-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.

Application Notes

The stated application concentrations are suggested starting points. Titration of the UDP-glucose 4-epimerase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 142-171 from the human protein was used as the immunogen for the UDP-glucose 4-epimerase antibody.

Storage

Aliquot the UDP-glucose 4-epimerase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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