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Home >> Antibodies >> UchL1 Antibody / PGP9.5

UchL1 Antibody / PGP9.5 [clone rUCHL1/8057] (V5030)

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  Catalog No Formulation Size Price (USD)  
Image V5030-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5030-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5030SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human cerebellum tissue with UchL1 antibody (clone rUCHL1/8057). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human cerebellum tissue with UchL1 antibody (clone rUCHL1/8057). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free UchL1 antibody (clone rUCHL1/8057) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rUCHL1/8057
Purity Protein A/G affinity
UniProt P09936
Localization Cytoplasm, ER membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This UchL1 antibody is available for research use only.
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Description

This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Application Notes

Optimal dilution of the UchL1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human UCHL1 protein was used as the immunogen for the UchL1 antibody.

Storage

Aliquot the UchL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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