UBXN1 Antibody / UBX domain-containing protein 1 (FY13448)

Western blot analysis of UBXN1 expression using UBXN1 antibody. UBXN1 expression was evaluated by western blotting in whole cell and tissue lysates separated by SDS-PAGE and transferred to a nitrocellulose membrane. Lanes were loaded with human HeLa, HepG2, 293T, and Ramos whole cell lysates, as well as rat liver and brain tissue lysates and mouse liver and brain tissue lysates. Immunodetection revealed a predominant UBXN1 band migrating at approximately 40 kDa across multiple samples, slightly higher than the predicted molecular weight of approximately 33 kDa. This upward shift is consistent with reported post-translational modification or altered electrophoretic mobility of UBXN1 under denaturing conditions.

Western blot analysis of UBXN1 expression using UBXN1 antibody. UBXN1 expression was examined by western blotting in whole cell lysates from human A431, Jurkat, K562, and SH-SY5Y cells, rat NRK and RH-35 cells, and mouse NIH/3T3 and HEPA1-6 cells. Proteins were separated by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane prior to immunodetection. A prominent UBXN1-immunoreactive band was observed at approximately 40 kDa across all tested samples, migrating higher than the predicted molecular weight of approximately 33 kDa. This apparent molecular weight shift is consistent with reported electrophoretic behavior of UBXN1, potentially reflecting post-translational modification or altered migration properties in SDS-PAGE systems.

IHC analysis of UBXN1 in human thyroid cancer tissue. Paraffin-embedded human thyroid cancer tissue was analyzed for UBXN1 expression by immunohistochemistry. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0), and sections were blocked with 10% goat serum prior to incubation with a rabbit anti-UBXN1 antibody overnight at 4°C. Detection was performed using an HRP-conjugated secondary antibody with DAB chromogen, followed by hematoxylin counterstaining. UBXN1 shows cytoplasmic staining within tumor cells.

IHC analysis of UBXN1 in human tonsil tissue. UBXN1 expression was examined in a paraffin-embedded section of human tonsil tissue using immunohistochemistry. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0). Sections were blocked with 10% goat serum and incubated with a rabbit anti-UBXN1 antibody overnight at 4°C. Detection was performed using an HRP-conjugated secondary antibody with DAB chromogen, and nuclei were counterstained with hematoxylin. UBXN1 staining is observed primarily in lymphoid cells with a cytoplasmic distribution.

IHC analysis of UBXN1 in human prostate hyperplasia tissue. UBXN1 expression was evaluated in a paraffin-embedded section of human prostate hyperplasia tissue. Heat-mediated antigen retrieval was carried out in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Tissue sections were incubated with a rabbit anti-UBXN1 antibody overnight at 4°C. Signal detection was achieved using an HRP-conjugated secondary antibody with DAB chromogen, with hematoxylin counterstaining. UBXN1 staining is evident in glandular epithelial cells.

IHC analysis of UBXN1 in mouse brain tissue. UBXN1 expression in mouse brain tissue was assessed using immunohistochemistry on paraffin-embedded sections. Antigen retrieval was performed using EDTA buffer (pH 8.0), and sections were blocked with 10% goat serum before overnight incubation with a rabbit anti-UBXN1 antibody at 4°C. Signal was visualized using an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining. UBXN1 staining is observed in neuronal cell populations.

IHC analysis of UBXN1 in rat brain tissue. Paraffin-embedded rat brain tissue sections were stained for UBXN1 using immunohistochemistry. Heat-mediated antigen retrieval was conducted in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Sections were incubated with a rabbit anti-UBXN1 antibody overnight at 4°C. Detection was achieved using an HRP-conjugated secondary antibody with DAB chromogen and hematoxylin counterstaining. UBXN1 immunoreactivity is present in neural tissue.

Immunoprecipitation of UBXN1 protein from 500ug of human HeLa whole cell lysate with 2ug of UBXN1 antibody.