UBR7 Antibody / Putative E3 ubiquitin-protein ligase UBR7 Antibody (FY13551)

UBR7 Antibody by IHC in rat testis. Immunohistochemistry of formalin-fixed, paraffin-embedded rat testis using Putative E3 ubiquitin-protein ligase UBR7 Antibody reveals brown immunoreactivity in cells within the seminiferous tubules. Positive nuclear staining is evident in subsets of germinal epithelial cells, with hematoxylin counterstaining providing blue nuclear contrast. Sections underwent heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and were incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen.

UBR7 Antibody by IP in human MCF-7 cell lysate. Immunoprecipitation using Putative E3 ubiquitin-protein ligase UBR7 Antibody enriches a protein migrating near 55 kDa from MCF-7 whole-cell lysate. The blot compares an input lysate, a rabbit IgG control immunoprecipitation and antibody-mediated immunoprecipitation. The immunoprecipitated band is detected using the same primary antibody and HRP-based chemiluminescence. The observed migration is above the calculated 48 kDa mass but falls within the 48-60 kDa range documented by independent antibody validation data. This experiment demonstrates recovery of an immunoreactive UBR7-associated protein from human cells.

UBR7 Antibody by IHC in mouse testis. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse testis using Putative E3 ubiquitin-protein ligase UBR7 Antibody demonstrates brown immunoreactivity within seminiferous tubules. Staining is concentrated in subsets of germinal epithelial cells, with variable nuclear labeling and blue hematoxylin counterstaining. Heat-induced epitope retrieval was performed in EDTA buffer at pH 8.0. Sections were incubated with 2 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and DAB detection. The image documents UBR7-associated immunoreactivity in mouse testicular tissue.

UBR7 Antibody by WB in human and rodent samples. Western blot analysis using Putative E3 ubiquitin-protein ligase UBR7 Antibody detects a band near 55 kDa in human MCF-7 (lane 1) and HeLa (lane 2) cells, rat testis (lane 3) and RH35 cells (lane 4), and mouse testis (lane 5) and NIH/3T3 cells (lane 6). The observed migration exceeds the calculated 48 kDa mass but is consistent with the 48-60 kDa range reported in independent antibody validation data. Reduced lysates containing 30 ug protein per lane were separated on 10% SDS-PAGE and probed with primary antibody at 0.5 ug/mL overnight at 4oC.