UBR2 Antibody / N-End Rule Pathway Regulator [clone PCRP-UBR2-1D12] (V8778)

UBR2 Antibody Human Thyroid IHC. Immunohistochemistry analysis of FFPE human thyroid tissue stained with clone PCRP-UBR2-1D12 at 2 ug/ml. Thyroid follicular epithelial cells display cytoplasmic and membranous HRP-DAB brown staining, while surrounding stromal elements show comparatively low background signal. The observed expression pattern is consistent with the involvement of Ubiquitin protein ligase E3 component n-recognin 2 / UBR2 in intracellular protein quality control and ubiquitin-mediated degradation pathways associated with cellular homeostasis. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UBR2 Antibody HeLa Cell FACS. Flow cytometry analysis of PFA-fixed human HeLa cells stained with clone PCRP-UBR2-1D12 (blue) and matched isotype control (red). The observed fluorescence shift demonstrates cellular detection of Ubiquitin protein ligase E3 component n-recognin 2 / UBR2, consistent with expression of this ubiquitin pathway regulator involved in N-end rule-mediated protein turnover and intracellular proteostasis signaling.

SDS-PAGE analysis of purified, BSA-free UBR2 Antibody / N-End Rule Pathway Regulator (PCRP-UBR2-1D12) as confirmation of integrity and purity.

UBR2 Antibody HeLa Cell IF. Immunofluorescent staining of PFA-fixed human HeLa cells using clone PCRP-UBR2-1D12 (green) and phalloidin cytoskeletal counterstain (red). Ubiquitin protein ligase E3 component n-recognin 2 / UBR2 displays predominantly cytoplasmic and perinuclear staining with additional punctate intracellular signal, consistent with its role in ubiquitin-mediated protein turnover and N-end rule pathway regulation associated with cellular proteostasis mechanisms.

UBR2 Antibody Protein Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using clone PCRP-UBR2-1D12. These results demonstrate high specificity of the mouse monoclonal antibody for Ubiquitin protein ligase E3 component n-recognin 2 / UBR2, a regulator of N-end rule-mediated protein degradation and ubiquitin-dependent proteostasis pathways. Z- and S-score analysis confirms strong preferential binding to the intended target relative to non-specific proteins represented on the HuProt(TM) array. The Z-score represents the signal strength generated by antibody binding to an individual protein relative to the overall array background, while the S-score reflects the relative specificity gap between the top-ranked target and subsequent proteins on the array.