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Home >> Antibodies >> Ubiquitin carboxyl-terminal hydrolase 16 Antibody / USP16

Ubiquitin carboxyl-terminal hydrolase 16 Antibody / USP16 (RQ7453)

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Image RQ7453 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HEL and 3) K562 cell lysate with Ubiquitin carboxyl-terminal hydrolase 16 antibody. Predicted molecular weight ~94 kDa.
Flow cytometry testing of human HepG2 cells with Ubiquitin carboxyl-terminal hydrolase 16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ubiquitin carboxyl-terminal hydrolase 16 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9Y5T5
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Ubiquitin carboxyl-terminal hydrolase 16 antibody is available for research use only.
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Description

Ubiquitin carboxyl-terminal hydrolase 16 is an enzyme that in humans is encoded by the USP16 gene. It is mapped to 21q21.3. This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Application Notes

Optimal dilution of the Ubiquitin carboxyl-terminal hydrolase 16 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids H103-Q683) was used as the immunogen for the Ubiquitin carboxyl-terminal hydrolase 16 antibody.

Storage

After reconstitution, the Ubiquitin carboxyl-terminal hydrolase 16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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