Ubiquitin C-terminal hydrolase L3 Antibody / UCHL3 (FY13451)

Western blot analysis of UCHL3 using UCHL3 antibody. Protein lysates from human HeLa cells (Lane 1), human Jurkat cells (Lane 2), human SW620 cells (Lane 3), human U-87MG cells (Lane 4), rat testis tissue (Lane 5), and mouse testis tissue (Lane 6) were resolved by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. UCHL3 was detected as a major band at approximately 26 kDa, consistent with the predicted molecular weight of Ubiquitin C-terminal hydrolase L3. A faint higher molecular weight band near 48 kDa may reflect ubiquitin-modified or ubiquitin-associated forms of UCHL3. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.

Western blot analysis of UCHL3 using UCHL3 antibody. Protein lysates from human HeLa cells (Lane 1), human Jurkat cells (Lane 2), human Caco-2 cells (Lane 3), human A431 cells (Lane 4), rat spleen tissue (Lane 5), rat brain tissue (Lane 6), mouse spleen tissue (Lane 7), and mouse brain tissue (Lane 8) were resolved by SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. UCHL3 was detected as a band at approximately 26 kDa, consistent with the predicted molecular weight of Ubiquitin C-terminal hydrolase L3. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.

Immunofluorescence analysis of UCHL3 using UCHL3 antibody and Alpha Tubulin antibody. UCHL3 expression was examined in cultured HeLa cells. Enzymatic antigen retrieval was performed prior to staining. Cells were blocked with normal goat serum and incubated with UCHL3 antibody (green) and Alpha Tubulin antibody (red) overnight at 4C. Immunoreactivity was visualized by fluorescence microscopy, showing UCHL3 localization in both the cytoplasm and nucleus, while Alpha Tubulin highlights the cytoskeletal network.

Immunoprecipitation and western blot analysis of UCHL3 using UCHL3 antibody. UCHL3 was immunoprecipitated from HeLa whole cell lysates using UCHL3 antibody and analyzed by SDS-PAGE followed by immunoblotting. Lane 1 shows HeLa whole cell lysate input, Lane 2 shows immunoprecipitation performed using rabbit control IgG, and Lane 3 shows immunoprecipitation performed using UCHL3 antibody. UCHL3 was detected as a band at approximately 26 kDa, consistent with the predicted molecular weight of Ubiquitin C-terminal hydrolase L3. The band observed near 50 kDa corresponds to the IgG heavy chain. Detection was performed using an HRP-based secondary antibody and chemiluminescent substrate.