UBE2G2 Antibody / Ubiquitin-conjugating enzyme E2 G2 Antibody (FY13535)

UBE2G2 Antibody by IHC in rat testis. Immunohistochemistry of paraffin-embedded rat testis using Ubiquitin-conjugating enzyme E2 G2 Antibody reveals brown staining in cells of the seminiferous tubules, with hematoxylin-counterstained nuclei. Heat-mediated antigen retrieval was performed in EDTA buffer at pH 8.0. After blocking with 10% goat serum, sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. Peroxidase-conjugated secondary antibody and DAB chromogen were used for visualization. The tissue image demonstrates UBE2G2-associated immunoreactivity within the testicular compartment.

UBE2G2 Antibody by WB in human and rodent lysates. Western blot analysis with Ubiquitin-conjugating enzyme E2 G2 Antibody detects a band near 17 kDa in HeLa (lane 1), K562 (lane 2), MCF-7 (lane 3), rat testis (lane 4), rat L6 cells (lane 5), mouse testis (lane 6), and NIH/3T3 cells (lane 7). The observed migration is close to the calculated molecular weight of approximately 18.6 kDa. Reduced lysates containing 30 ug protein per lane were resolved by 12% SDS-PAGE and transferred to nitrocellulose. Primary antibody was applied at 0.5 ug/mL overnight at 4oC, followed by HRP-linked secondary antibody and chemiluminescent detection.

UBE2G2 Antibody by FACS in mouse 3T3-L1 cells. Flow cytometric analysis using Ubiquitin-conjugating enzyme E2 G2 Antibody shows a rightward shift of stained 3T3-L1 cells (blue trace) relative to the rabbit IgG isotype control (green) and unstained cells (red). Cells were fixed in 4% paraformaldehyde and permeabilized before blocking with 10% goat serum. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by DyLight 488-conjugated secondary antibody. The histogram demonstrates an antibody-associated intracellular fluorescence signal.

UBE2G2 Antibody by FACS in RT4 cells. Flow cytometric analysis using Ubiquitin-conjugating enzyme E2 G2 Antibody demonstrates increased fluorescence in stained RT4 cells (blue trace) compared with the isotype control (green) and unstained control (red). Cells were fixed with 4% paraformaldehyde, permeabilized, and blocked with 10% goat serum. Primary antibody was incubated at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. DyLight 488-conjugated secondary antibody was used for detection. This second cell model provides complementary evidence of antibody-associated UBE2G2 staining.