UAP1L1 Antibody / UDP-GlcNAc Pyrophosphorylase Antibody (FY13515)

UAP1L1 Antibody by IF in Human MCF-7 Breast Cancer Cells. Immunofluorescence staining of human MCF-7 cells using rabbit polyclonal UAP1L1 antibody at 5 ug/ml. Enzymatic antigen retrieval was performed for 15 minutes, followed by blocking with 10% goat serum and overnight primary antibody incubation at 4oC. Cy3-conjugated goat anti-rabbit IgG was applied at 1:500 for 30 minutes at 37oC, and nuclei were counterstained with DAPI. Red fluorescence demonstrates UDP-GlcNAc Pyrophosphorylase-related protein UAP1L1 immunoreactivity, predominantly distributed throughout the cytoplasm with a diffuse, finely granular appearance. The blue DAPI-stained nuclei exhibit comparatively little red fluorescence.

UAP1L1 Antibody by WB in Human Cell Lines and Rodent Kidney Tissue. Western blot analysis of human THP-14, OE19, Caco-2, and MCF-7 whole-cell lysates, rat kidney tissue lysate, and mouse kidney tissue lysate using rabbit polyclonal UAP1L1 antibody at 0.5 ug/ml. Lanes 1-6 correspond to THP-14, OE19, Caco-2, MCF-7, rat kidney, and mouse kidney, respectively. Samples containing 30 ug of protein per lane were separated by 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. Following overnight primary antibody incubation at 4oC, detection was performed using HRP-conjugated goat anti-rabbit IgG at 1:5000 and chemiluminescence. A prominent band is detected at approximately 57 kDa in all six samples, consistent with the predicted molecular weight of UDP-GlcNAc Pyrophosphorylase-related protein UAP1L1. Weaker additional bands are visible near 70 kDa in several lanes.