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Home >> Antibodies >> Tyrosine Hydroxylase Antibody

Tyrosine Hydroxylase Antibody [clone TH-16] (R30089)

  Catalog No Formulation Size Price (USD)  
Image R30089 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS 100 ug 429
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Western blot testing of 1) rat brain, 2) mouse brain and 3) human U-87 MG cell lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight ~59 kDa.
IHC staining of FFPE rat brain tissue with Tyrosine Hydroxylase antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Ascites
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name TH-16
Purity Unpurified ascites
Gene ID 7054
Applications Western blot : 0.25-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml; formalin or acetone fixed tissues
Limitations This Tyrosine Hydroxylase antibody is available for research use only.
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Description

Tyrosine hydroxylase (TH) is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, TH has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Tyrosine Hydroxylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Rat tyrosine hydroxylase was used as the immunogen for this Tyrosine Hydroxylase antibody.

Storage

After reconstitution, the Tyrosine Hydroxylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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