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Home >> Antibodies >> Tyrosine Hydroxylase Antibody / TH

Tyrosine Hydroxylase Antibody / TH [clone ECA-20] (RQ5166)

  Catalog No Formulation Size Price (USD)  
Image RQ5166 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Immunofluorescent staining of FFPE rat PC-12 cells with Tyrosine Hydroxylase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human PC-3 cell lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight: 55-60 kDa.
Western blot testing of human SK-BR-3 cell lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight: 55-60 kDa.
Western blot testing of 1) mouse heart, 2) mouse brain, 3) rat heart and 4) rat lung tissue lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight: 55-60 kDa.
Western blot testing of mouse penis tissue lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight: 55-60 kDa.
Western blot testing of rat brain tissue lysate with Tyrosine Hydroxylase antibody. Predicted molecular weight: 55-60 kDa.
IHC staining of FFPE human kidney tissue with Tyrosine Hydroxylase antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name ECA-20
Purity Affinity purified
UniProt P07101
Localization Nuclear, cytoplasmic, cell membrane
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence : 1:50-1200
Limitations This Tyrosine Hydroxylase antibody is available for research use only.
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Description

The protein encoded by the TH gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome.

Application Notes

Optimal dilution of the Tyrosine Hydroxylase antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Tyrosine Hydroxylase / TH was used as the immunogen for the Tyrosine Hydroxylase antibody.

Storage

Store the Tyrosine Hydroxylase antibody at -20oC.

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