TYMS Antibody / Thymidylate Synthase [clone TYMS/1884] (V3515)

Description

This antibody recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS, TYMS). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Application Notes

The optimal dilution of the TYMS antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human parital protein corresponding to amino acids 60-174 was used as the immunogen for this TYMS antibody.

Storage

Store the TYMS antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).