TYMP Antibody / Thymidine phosphorylase / PD-ECGF (FY13337)

TYMP Antibody SiHa IP-WB. Immunoprecipitation of TYMP from human SiHa whole cell lysate followed by western blot detection using TYMP antibody. Lane 1: SiHa whole cell lysate (input), Lane 2: control IgG immunoprecipitation, Lane 3: TYMP immunoprecipitation using TYMP antibody. The input lane shows a characteristic doublet at approximately 45-55 kDa, while the immunoprecipitated sample displays a predominant band at approximately 50 kDa, consistent with enrichment of the full-length TYMP protein. The absence of signal in the control IgG lane confirms specificity of the immunoprecipitation. The observed banding pattern aligns with TYMP as a cytosolic enzyme involved in nucleotide metabolism and angiogenesis-associated signaling.

TYMP Antibody Multi-Sample WB. Western blot analysis of human whole cell lysates using TYMP antibody detecting thymidine phosphorylase. Lane 1: human whole cell lysate, Lane 2: SiHa whole cell lysate, Lane 3: THP-1 whole cell lysate. A predominant band is detected at approximately 50 kDa in all samples, slightly below the predicted ~55 kDa molecular weight but consistent with the known migration behavior of TYMP. A weaker band is also observed in the high 40 kDa range, likely representing a processed or partially cleaved form of the protein. The consistent detection across samples aligns with TYMP expression as a cytosolic enzyme involved in nucleotide metabolism and angiogenesis-associated signaling.

TYMP Antibody A431 FACS. Flow cytometry analysis of human A431 cells stained with TYMP antibody detecting thymidine phosphorylase. The antibody signal (blue) shows a clear rightward shift compared to the blank control (red) and isotype control (green), indicating positive intracellular detection of TYMP following fixation and permeabilization. This staining profile is consistent with TYMP as a cytosolic enzyme involved in nucleotide metabolism and angiogenesis-associated signaling.

TYMP Antibody SiHa FACS. Flow cytometry analysis of human SiHa cells stained with TYMP antibody detecting thymidine phosphorylase. The antibody signal (blue) shows a clear rightward shift compared to the blank control (red) and isotype control (green), indicating positive intracellular detection of TYMP following fixation and permeabilization. This staining pattern is consistent with TYMP as a cytosolic enzyme involved in nucleotide metabolism and angiogenesis-associated signaling.