TXNRD1 Antibody / Thioredoxin reductase 1 (FY13051)

Western blot analysis of TXNRD1 using anti-TXNRD1 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A strong band is detected at ~65 kDa, with a weaker band at ~60 kDa, while the theoretical molecular weight of TXNRD1 is ~71 kDa. This downward shift is commonly reported and reflects the migration properties of the cytosolic selenoprotein, which often runs ~5-10 kDa lower than predicted. The two bands likely correspond to the major TXNRD1 isoform (~65 kDa) and a C-terminally truncated variant (~60 kDa) lacking the selenocysteine-containing tail.