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Home >> Antibodies >> TXN2 Antibody / Thioredoxin 2

TXN2 Antibody / Thioredoxin 2 [clone 7B5] (RQ5639)

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  Catalog No Formulation Size Price (USD)  
Image RQ5639 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with TXN2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with TXN2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HL-60 cells with TXN2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TXN2 antibody.
Western blot testing of human 1) HeLa, 2) Raji, 3) K562, 4) A549, 5) Caco-2, 6) ThP-1, 7) rat PC-12 and 8) mouse RAW264.7 lysate with TXN2 antibody. Predicted molecular weight ~13 kDa.
IHC staining of FFPE human lung cancer with TXN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human stomach cancer with TXN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with TXN2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Name 7B5
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q99757
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This TXN2 antibody is available for research use only.
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Description

Thioredoxin, mitochondrial also known as thioredoxin-2 is a protein that in humans is encoded by the TXN2 gene on chromosome 22. It is mapped to 22q12.3. This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

Application Notes

Optimal dilution of the TXN2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids T60-G166) was used as the immunogen for the TXN2 antibody.

Storage

After reconstitution, the TXN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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