Tumor Suppressor p53 Antibody / TP53 [clone rIMX25] (V6014)

Western blot analysis of Tumor Suppressor p53 / TP53 antibody in human A431 cell lysate (clone rTP53/13508). A distinct band is observed at approximately 53 kDa, consistent with the predicted molecular weight of Tumor protein 53. The detected band corresponds to endogenous p53 expressed in A431 cells. The migration pattern aligns with the expected size of full length TP53 under reducing SDS-PAGE conditions.

Immunohistochemistry analysis of Tumor Suppressor p53 / TP53 antibody in human tonsil tissue (clone rIMX25). FFPE tonsil sections demonstrate nuclear HRP-DAB brown staining in epithelial cells lining crypt structures, while surrounding stromal and lymphoid areas show minimal to weak background signal. The staining pattern is predominantly nuclear, consistent with the role of Tumor protein 53 as a transcription factor regulating cell cycle and DNA damage responses. Lymphoid cells within germinal center regions show limited nuclear signal under physiologic conditions. The inset image shows the PBS secondary-only negative control, confirming absence of specific staining in the control section. Heat induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95C followed by cooling at room temperature prior to primary antibody incubation.

SDS-PAGE Analysis of Purified Tumor Suppressor p53/TP53 antibody (rIMX25). Confirmation of Purity and Integrity of Antibody.