TUBA1A Antibody / Neuronal Microtubule Protein Antibody (RQ5776)

TUBA1A Antibody A431 Cell IF. Immunofluorescent staining of FFPE human A431 cells using TUBA1A Antibody demonstrates strong red cytoplasmic staining with a filamentous network pattern consistent with localization of Alpha Tubulin (TUBA1A) within the cellular microtubule cytoskeleton. TUBA1A is a highly conserved microtubule-associated protein that serves as a major structural component of microtubules, which regulate cellular architecture, intracellular transport, cell polarity, and cytoskeletal organization. The extensive filamentous staining highlights the microtubule network throughout the cytoplasm, while DAPI (blue) counterstains cell nuclei. HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes prior to staining.

TUBA1A Antibody Rat NRK Cell IF. Immunofluorescent staining of FFPE rat NRK cells using TUBA1A Antibody demonstrates strong red cytoplasmic staining with a filamentous distribution consistent with localization of Alpha Tubulin (TUBA1A) within the cellular microtubule cytoskeleton. TUBA1A is a highly conserved microtubule-associated protein that functions as a major structural component of microtubules, supporting cellular architecture, intracellular transport, cytoskeletal organization, and cell polarity. The staining pattern highlights microtubule-rich regions extending throughout the cytoplasm and cellular processes, while DAPI (blue) counterstains cell nuclei. HIER: steam tissue sections in pH 6 citrate buffer for 20 minutes prior to staining.

TUBA1A Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placenta tissue using TUBA1A Antibody demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within placental trophoblast and epithelial-associated cellular populations. The staining pattern is consistent with expression of Alpha Tubulin (TUBA1A), a highly conserved microtubule-associated protein that serves as a major structural component of the intracellular microtubule cytoskeleton. TUBA1A immunoreactivity highlights cellular architecture and microtubule-rich cytoplasmic compartments throughout placental tissue, supporting its role in intracellular transport, cytoskeletal organization, and maintenance of cellular structure. HIER: boil tissue sections in pH 8 EDTA buffer for 20 minutes and allow to cool before testing.

TUBA1A Antibody Multi-Species Western Blot. Western blot analysis of human HeLa (lane 1), Jurkat (lane 2), HEK293 (lane 3), HepG2 (lane 4), SW620 (lane 5), Raji (lane 6), K562 (lane 7), rat brain (lane 8), and mouse brain (lane 9) lysates using TUBA1A Antibody demonstrates a prominent band at approximately 50 kDa, consistent with the predicted molecular weight of Alpha Tubulin (TUBA1A). TUBA1A is a highly conserved microtubule-associated protein that serves as a major structural component of the intracellular microtubule cytoskeleton, where it supports cellular architecture, intracellular transport, cytoskeletal organization, and neuronal function. Detection of the expected molecular weight band across multiple human cell lines and rodent brain tissues supports the conserved expression of TUBA1A and the suitability of this antibody for western blot applications.

TUBA1A Antibody HeLa Cell FACS. Flow cytometry analysis of human HeLa cells using TUBA1A Antibody demonstrates a pronounced rightward shift of the positive cell population (blue) relative to the isotype control (green), consistent with expression of Alpha Tubulin (TUBA1A). TUBA1A is a highly conserved microtubule-associated protein that serves as a major structural component of the intracellular microtubule cytoskeleton, supporting cellular architecture, intracellular transport, cytoskeletal organization, and cell division. Cells were blocked with goat serum prior to staining. Red=cells alone; Green=isotype control; Blue=TUBA1A antibody.