TTF-1 Antibody / Thyroid Tumor Marker Antibody [clone NX2.1/690] (V2894)

Description

Thyroid transcription factor 1 (NKX2-1) is a nuclear homeobox transcription factor that regulates epithelial lineage specification in the thyroid gland, lung, and forebrain. The protein functions as a DNA-binding transcriptional regulator controlling genes required for thyroid follicular epithelial differentiation and endocrine gland development. The TTF-1 Antibody / Thyroid Tumor Marker Antibody targets this lineage-defining transcription factor, which localizes to the nuclei of thyroid follicular epithelial cells and plays a central role in maintaining thyroid epithelial identity and endocrine lineage commitment.

In endocrine pathology, TTF-1 antibody staining is widely used as a thyroid tumor marker because NKX2-1 expression is strongly associated with thyroid follicular epithelial cells and thyroid-derived tumors. Nuclear staining for Thyroid transcription factor 1 is frequently observed in thyroid neoplasms, including papillary thyroid carcinoma and follicular thyroid carcinoma. Because of this characteristic nuclear staining pattern, TTF-1 antibody immunohistochemistry is commonly used to confirm thyroid epithelial lineage and to support the diagnosis of primary thyroid tumors. Clone NX2.1/690 antibody is a mouse monoclonal antibody designed to detect nuclear NKX2-1 protein in thyroid epithelial cells and thyroid carcinoma specimens.

TTF-1 antibody, also referred to as NKX2-1 antibody or Thyroid transcription factor 1 antibody in the literature, recognizes a transcription factor that regulates genes involved in thyroid hormone synthesis and endocrine epithelial differentiation. NKX2-1 activates thyroid-specific genes including thyroglobulin and thyroid peroxidase, which are essential for thyroid hormone biosynthesis and thyroid follicular cell function. Through these regulatory activities, the transcription factor helps establish the specialized endocrine phenotype of thyroid follicular epithelial cells and supports thyroid gland development.

In surgical pathology practice, TTF-1 antibody staining patterns are particularly valuable when evaluating thyroid tumors. Strong nuclear immunoreactivity supports thyroid follicular epithelial differentiation and helps distinguish primary thyroid carcinoma from metastatic carcinomas involving the thyroid gland. For this reason, TTF-1 antibody is commonly included in immunohistochemical tumor panels used for endocrine tumor evaluation and thyroid carcinoma diagnosis.

Because NKX2-1 remains tightly associated with thyroid epithelial lineage, the protein is widely regarded as an important thyroid tumor marker and endocrine epithelial differentiation marker. A TTF-1 antibody such as clone NX2.1/690 provides a valuable tool for research focused on thyroid carcinoma biology, thyroid epithelial differentiation, and transcriptional regulation of endocrine epithelial lineage identity.

Application Notes

Optimal dilution of the TTF-1 Antibody / Thyroid Tumor Marker Antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for the TTF-1 antibody.

Storage

Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NKX2-1 antibody, Thyroid transcription factor 1 antibody, TTF1 antibody, TITF1 antibody, Thyroid transcription factor antibody