TTF-1 Antibody / Pulmonary Epithelial Cell Marker Antibody [clone HBNK2-1] (V7084)

Description

Thyroid transcription factor 1 (NKX2-1) is a nuclear homeobox transcription factor that regulates epithelial lineage development in the lung, thyroid, and forebrain. The protein functions as a DNA-binding transcriptional regulator controlling genes required for respiratory epithelial differentiation and pulmonary tissue development. The TTF-1 Antibody / Pulmonary Epithelial Cell Marker Antibody targets this lineage-defining transcription factor, which localizes to the nuclei of pulmonary epithelial cells and plays a central role in establishing pulmonary epithelial lineage identity within the respiratory epithelium.

In lung biology, TTF-1 antibody staining is widely used as a pulmonary epithelial cell marker because NKX2-1 expression is strongly associated with respiratory epithelial cells. Nuclear staining for Thyroid transcription factor 1 is commonly observed in alveolar epithelial cells, particularly type II pneumocytes, as well as bronchiolar epithelial cells that form the lining of the respiratory epithelium. Because of this consistent nuclear expression pattern, TTF-1 antibody immunohistochemistry is frequently used to identify pulmonary epithelial lineage and to study respiratory epithelial cell populations within lung tissue.

TTF-1 antibody, also referred to as NKX2-1 antibody or Thyroid transcription factor 1 antibody in the literature, recognizes a transcription factor that regulates multiple genes required for pulmonary epithelial cell function. NKX2-1 activates lung-specific genes including surfactant proteins and other molecules essential for alveolar epithelial physiology and gas exchange. Through these regulatory activities, the transcription factor supports differentiation of alveolar epithelial cells and helps maintain the structural and functional integrity of the respiratory epithelium.

During embryonic development, NKX2-1 is expressed early within the respiratory diverticulum and plays a critical role in lung bud formation and branching morphogenesis. This early expression establishes NKX2-1 as a master regulator of pulmonary epithelial lineage specification. As lung tissue matures, the transcription factor remains highly expressed in respiratory epithelial cells, particularly alveolar epithelial cells, reinforcing its role as a defining marker of pulmonary epithelial lineage within the respiratory epithelium.

Because NKX2-1 expression remains tightly associated with pulmonary epithelial cells, the protein is widely regarded as an important pulmonary epithelial cell marker and respiratory epithelial lineage marker used in lung biology research. A TTF-1 antibody such as clone HBNK2-1 provides a valuable tool for studies investigating pulmonary epithelial differentiation, alveolar epithelial cell biology, and transcriptional regulation of genes required for respiratory epithelial function.

Application Notes

Titering of the TTF-1 Antibody / Pulmonary Epithelial Cell Marker Antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for the TTF-1 antibody.

Storage

Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NKX2-1 antibody, Thyroid transcription factor 1 antibody, TTF1 antibody, TITF1 antibody, Thyroid transcription factor antibody