TTF-1 Antibody Clone 8G7G3/1 / NKX2.1 [clone 8G7G3/1] (V2274)

Description

Thyroid transcription factor 1 (NKX2-1) is a nuclear homeobox transcription factor that regulates epithelial lineage development in the lung, thyroid gland, and forebrain. The protein functions as a DNA-binding transcriptional regulator controlling genes involved in epithelial differentiation and organ development. TTF-1 Antibody Clone 8G7G3/1 targets this lineage-defining transcription factor, which localizes to the nucleus where it regulates transcriptional programs that establish epithelial lineage identity in respiratory epithelium and thyroid follicular epithelium.

TTF-1 antibody, also referred to as NKX2-1 antibody or Thyroid transcription factor 1 antibody in the literature, detects a nuclear transcription factor expressed in pulmonary epithelial cells and thyroid follicular epithelial cells. Clone 8G7G3/1 antibody is one of the most widely recognized monoclonal antibodies used to detect NKX2-1 in immunohistochemistry and has been extensively used in studies examining lung and thyroid epithelial lineage markers. Because NKX2-1 localizes to the nucleus, TTF-1 antibody staining typically produces strong nuclear immunoreactivity in epithelial cells expressing this transcription factor.

In lung tissue, NKX2-1 regulates genes associated with respiratory epithelial differentiation, including surfactant proteins expressed by alveolar epithelial cells. Nuclear expression of TTF-1 is commonly observed in pulmonary adenocarcinoma and small cell lung carcinoma, making TTF-1 clone 8G7G3/1 antibody an important marker in immunohistochemical tumor panels used for evaluating lung tumors and identifying pulmonary epithelial origin.

In thyroid tissue, NKX2-1 regulates genes involved in thyroid hormone biosynthesis and supports differentiation of thyroid follicular epithelial cells. Nuclear staining with clone 8G7G3/1 antibody highlights thyroid follicular epithelial lineage and helps confirm tumors derived from thyroid epithelial cells in diagnostic pathology. Because NKX2-1 expression is associated with epithelial cells of lung and thyroid origin, TTF-1 antibody staining is widely used in surgical pathology tumor panels to assist in determining tumor lineage.

Due to its consistent nuclear staining pattern and extensive use in published research, clone 8G7G3/1 antibody remains one of the most widely cited monoclonal antibodies for detecting Thyroid transcription factor 1. A TTF-1 antibody such as clone 8G7G3/1 provides a valuable tool for studies focused on epithelial lineage biology, transcription factor localization, and diagnostic evaluation of lung and thyroid tumors.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TTF-1 Antibody Clone 8G7G3/1 to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Rat full length recombinant protein was used as the immunogen for this TTF-1 antibody.

Storage

Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NKX2-1 antibody, Thyroid transcription factor 1 antibody, TTF1 antibody, TITF1 antibody, Thyroid transcription factor antibody