TTF-1 Antibody / Lung and Thyroid Diagnostic Marker Antibody [clone 8G7G3/1 + NX2.1/690] (V2895)

Description

Thyroid transcription factor 1 (NKX2-1) is a nuclear homeobox transcription factor that regulates epithelial lineage development in the lung and thyroid gland. The protein functions as a DNA-binding transcriptional regulator controlling genes required for pulmonary epithelial differentiation and thyroid follicular epithelial development. The TTF-1 Antibody / Lung and Thyroid Diagnostic Marker Antibody targets this lineage-defining transcription factor, which localizes to the nuclei of epithelial cells within respiratory epithelium and thyroid follicular epithelium.

In surgical pathology, TTF-1 antibody staining is widely used as a lung and thyroid diagnostic marker in immunohistochemistry panels. Nuclear immunoreactivity for Thyroid transcription factor 1 is frequently observed in pulmonary adenocarcinoma and small cell lung carcinoma, as well as in tumors derived from thyroid follicular epithelium. Because NKX2-1 expression is strongly associated with epithelial cells of lung and thyroid origin, TTF-1 antibody staining is routinely used to help determine tumor origin in diagnostic pathology.

TTF-1 antibody, also referred to as NKX2-1 antibody or Thyroid transcription factor 1 antibody in the literature, plays an important role in immunohistochemical tumor panels used to classify carcinomas. In lung tumor diagnostics, nuclear TTF-1 staining supports pulmonary epithelial origin and helps distinguish primary lung adenocarcinoma from metastatic carcinomas involving the lung. In thyroid pathology, nuclear staining highlights thyroid follicular epithelial lineage and assists in confirming tumors arising from thyroid epithelial cells.

Because of its highly characteristic nuclear staining pattern, TTF-1 antibody is frequently incorporated into diagnostic immunohistochemistry panels together with markers such as Napsin A, cytokeratins, and other epithelial lineage markers when evaluating lung tumors. Similarly, TTF-1 antibody staining may be used alongside thyroid markers such as thyroglobulin and PAX8 when assessing thyroid tumors. These diagnostic marker panels allow pathologists to determine tumor lineage and identify the likely tissue of origin in carcinomas.

Clones 8G7G3/1 and NX2.1/690 antibodies are mouse monoclonal antibodies developed to detect nuclear NKX2-1 protein in epithelial cells derived from lung and thyroid tissues. A TTF-1 antibody such as clones 8G7G3/1 + NX2.1/690 therefore provides a valuable diagnostic immunohistochemistry marker for studies focused on tumor origin identification, lung carcinoma classification, and thyroid tumor pathology.

Application Notes

Optimal dilution of the TTF-1 Antibody / Lung and Thyroid Diagnostic Marker Antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for the TTF-1 antibody cocktail.

Storage

Store the TTF-1 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

NKX2-1 antibody, Thyroid transcription factor 1 antibody, TTF1 antibody, TITF1 antibody, Thyroid transcription factor antibody