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Home >> Antibodies >> TSPAN12 Antibody

TSPAN12 Antibody (RQ4142)

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  Catalog No Formulation Size Price (USD)  
Image RQ4142 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human HeLa and 2) human SW620 cell lysate with TSPAN12 antibody at 0.5ug/ml. Predicted molecular weight ~35 kDa, may be observed at a higher MW due to glycosylation.
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Western blot testing of 1) human HeLa and 2) human SW620 cell lysate with TSPAN12 antibody at 0.5ug/ml. Predicted molecular weight ~35 kDa, may be observed at a higher MW due to glycosylation.
IHC testing of FFPE human placental tissue with TSPAN12 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human lung cancer tissue with TSPAN12 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human rectal cancer tissue with TSPAN12 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse lung tissue with TSPAN12 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lung tissue with TSPAN12 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of 1) rat kidney, 2) rat brain and 3) mouse brain lysate with TSPAN12 antibody at 0.5ug/ml. Predicted molecular weight ~35 kDa, may be observed at a higher MW due to glycosylation.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O95859
Localization Cytoplasm, cell membrane
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This TSPAN12 antibody is available for research use only.
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Description

Tetraspanin-12 (Tspan-12) also known as tetraspan NET-2 (NET2) or transmembrane 4 superfamily member 12 (TM4SF12) is a tetraspanin protein that in humans is encoded by the TSPAN12 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.

Application Notes

Optimal dilution of the TSPAN12 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein corresponding to amino acids G111-R224 was used as the immunogen for the TSPAN12 antibody.

Storage

After reconstitution, the TSPAN12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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