TSPAN12 Antibody (RQ4142)

Description

TSPAN12, or tetraspanin 12, is a member of the tetraspanin family of transmembrane proteins that are involved in a wide array of cellular processes, including signal transduction, cell adhesion, and proliferation. TSPAN12 is best known for its role in regulating the Norrin/beta-catenin signaling pathway, which is essential for retinal vascular development and maintenance of the blood-retinal barrier.

TSPAN12 is expressed in various tissues, with prominent roles in vascular endothelial cells and neural tissues. Genetic mutations in TSPAN12 are linked to familial exudative vitreoretinopathy, a hereditary disorder characterized by incomplete development of retinal vasculature. Several isoforms of TSPAN12 have been identified, arising from alternative splicing events. These isoforms may differ in their subcellular localization and regulatory functions, adding complexity to its role in health and disease.

The TSPAN12 antibody is a powerful tool for detecting endogenous protein levels in both normal and pathological tissues. A validated TSPAN12 antibody can be employed in immunohistochemistry, western blotting, and immunofluorescence to monitor expression changes or investigate its role in angiogenesis and retinal disorders. When used in experimental studies, the TSPAN12 antibody provides critical insights into the regulation of vascular signaling and tissue integrity.

NSJ Bioreagents offers high-quality TSPAN12 antibody products designed for robust performance across multiple assay types, enabling researchers to confidently explore the function of this important protein in biological and disease-related contexts.

Application Notes

Optimal dilution of the TSPAN12 antibody should be determined by the researcher.

Immunogen

A recombinant human partial protein corresponding to amino acids G111-R224 was used as the immunogen for the TSPAN12 antibody.

Storage

After reconstitution, the TSPAN12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.