TSH Receptor Antibody / TSHR (R32688)

IHC analysis of TSH Receptor Antibody / TSHR. TSH Receptor expression was examined in a paraffin-embedded section of human thyroid cancer tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with goat serum and incubated with a rabbit anti-TSH Receptor antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. Staining is primarily observed along the membranes of thyroid follicular epithelial cells, with additional cytoplasmic signal consistent with receptor synthesis and intracellular trafficking.

Western blot analysis of TSH Receptor Antibody / TSHR. Proteins were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane for immunodetection. Lane 1: human Jurkat whole cell lysates; Lane 2: human REH whole cell lysates; Lane 3: human K562 whole cell lysates; Lane 4: human TT whole cell lysates; Lane 5: rat ovary tissue lysates; Lane 6: mouse ovary tissue lysates; Lane 7: mouse thymus tissue lysates. Multiple immunoreactive bands corresponding to TSHR are observed, including species migrating at approximately 40 kDa, 70-80 kDa, and 110 kDa. The predicted molecular weight of full-length TSHR is approximately 87 kDa based on amino acid sequence. The higher molecular weight band is consistent with glycosylated full-length receptor, while lower molecular weight bands likely represent proteolytic cleavage products and partially processed receptor forms. Such banding heterogeneity is well documented for TSHR due to extensive N-linked glycosylation, extracellular domain cleavage, and variable post-translational processing across tissues and cell types.