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Home >> Antibodies >> Tryptophan Hydroxylase Antibody / TPH1

Tryptophan Hydroxylase Antibody / TPH1 (F54902)

  Catalog No Formulation Size Price (USD)  
Image F54902-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54902-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human HEK293 cell lysate with Tryptophan Hydroxylase antibody. Predicted molecular weight ~51 kDa.
IHC testing of FFPE human brain tissue with Tryptophan Hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human ThP1 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human WiDr cells with Tryptophan Hydroxylase antibody; Blue=isotype control, Green= Tryptophan Hydroxylase antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P17752
Localization Cytoplasmic
Applications Immunofluorescence : 1:10-1:50
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500
Limitations This Tryptophan Hydroxylase antibody is available for research use only.
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Description

TPH1 is a member of the aromatic amino acid hydroxylase family. This protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Tryptophan Hydroxylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 35-62 from the human protein was used as the immunogen for the Tryptophan Hydroxylase antibody.

Storage

Aliquot the Tryptophan Hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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