TRPV5 Antibody (RQ5559)

IHC staining of FFPE human pancreatic cancer with TRPV5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE mouse kidney with TRPV5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat kidney with TRPV5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Western blot testing of 1) monkey COS-7, 2) human U-87 MG, 3) human HeLa and 4) human HepG2 lysate with TRPV5 antibody. Western blot testing of human pancreas lysate with TRPV5 antibody at 1ug/ml. Predicted/observed molecular weight: ~85 kDa.

Flow cytometry testing of human U-87 MG cells with TRPV5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRPV5 antibody.

Flow cytometry testing of human HepG2 cells with TRPV5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRPV5 antibody.