TRIO Antibody / Triple functional domain protein Antibody (FY13516)

TRIO Antibody by IF in human lung carcinoma. Immunofluorescence staining of paraffin-embedded human lung carcinoma using Triple functional domain protein Antibody reveals prominent red signal surrounding DAPI-stained blue nuclei, outlining epithelial structures and cellular processes. Tissue underwent heat-mediated antigen retrieval in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The primary antibody was applied at 5 ug/mL overnight at 4oC, followed by a Cy3-conjugated anti-rabbit secondary antibody at 1:500. The image demonstrates abundant TRIO-associated immunoreactivity across the tumor tissue.

TRIO Antibody by IHC in human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human lung carcinoma using Triple functional domain protein Antibody demonstrates heterogeneous brown staining in tumor cells, with a predominantly cytoplasmic distribution and blue nuclear counterstaining. Antigen retrieval was performed using EDTA buffer, pH 8.0. Sections were blocked with 10% goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC. An HRP-conjugated anti-rabbit detection system and DAB chromogen were used to visualize immunoreactivity. The image shows variation in staining intensity across the tumor field.

TRIO Antibody by FACS in U2OS cells. Flow cytometry analysis of fixed U2OS cells using Triple functional domain protein Antibody shows a rightward shift in fluorescence intensity for the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unstained blank (red). Cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and incubated with 1 ug antibody per 1 x 10^6 cells. A fluorescent anti-rabbit secondary antibody was used for detection. The separation of the distributions supports intracellular TRIO-associated staining in this cell line.

TRIO Antibody by IF in human breast carcinoma. Immunofluorescence staining of paraffin-embedded human breast carcinoma using Triple functional domain protein Antibody shows red TRIO-associated immunoreactivity in groups of tumor cells, with DAPI-stained nuclei appearing blue. Staining varies across the field and is visible around numerous nuclei. Sections underwent heat-mediated EDTA antigen retrieval at pH 8.0 and blocking with goat serum. The primary antibody was applied at 5 ug/mL overnight at 4oC, followed by a Cy3-conjugated anti-rabbit secondary antibody. The image illustrates the distribution of the detected antigen within breast carcinoma tissue.

TRIO Antibody by IHC in human breast carcinoma. Immunohistochemistry of paraffin-embedded human breast carcinoma using Triple functional domain protein Antibody shows brown immunoreactivity in scattered tumor cells and surrounding tissue, with blue hematoxylin-counterstained nuclei. The intensity of staining differs among cells and tissue regions. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer, pH 8.0, blocked with 10% goat serum, and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection employed an HRP-linked anti-rabbit system and DAB chromogen. The image documents heterogeneous TRIO-associated staining in this tissue.