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Home >> Antibodies >> TRIM5 Antibody

TRIM5 Antibody (RQ4369)

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  Catalog No Formulation Size Price (USD)  
Image RQ4369 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human A375 cell lysate with TRIM5 antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa.
Western blot testing of human A375 cell lysate with TRIM5 antibody at 0.5ug/ml. Predicted molecular weight ~56 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9C035
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This TRIM5 antibody is available for research use only.
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Description

Tripartite motif-containing protein 5 also known as RING finger protein 88 is a protein that in humans is encoded by the TRIM5 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Application Notes

Optimal dilution of the TRIM5 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids Y62-N307 was used as the immunogen for the TRIM5 antibody.

Storage

After reconstitution, the TRIM5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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