TRIM46 Antibody / Axon Initial Segment Protein Antibody (FY13464)

TRIM46 Antibody / Axon Initial Segment Protein Antibody Human Cerebral Cortex IHC. Immunohistochemical staining of FFPE human cerebral cortex tissue using TRIM46 Antibody / Axon Initial Segment Protein Antibody. TRIM46 expression is detected predominantly within neuronal processes and cell bodies, consistent with its role in organizing parallel microtubule bundles at the axon initial segment and establishing neuronal polarity. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

TRIM46 Antibody / Axon Initial Segment Protein Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue using TRIM46 Antibody / Axon Initial Segment Protein Antibody. TRIM46 expression is detected predominantly within neuronal processes, consistent with its localization to the axon initial segment where it organizes parallel microtubule bundles and establishes neuronal polarity. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

TRIM46 Antibody / Axon Initial Segment Protein Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using TRIM46 Antibody / Axon Initial Segment Protein Antibody. TRIM46 expression is detected predominantly within axon initial segments of neurons, consistent with its role in organizing parallel microtubule bundles that establish neuronal polarity and support axonal identity. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

TRIM46 Antibody / Axon Initial Segment Protein Antibody Human, Mouse and Rat WB. Western blot analysis of 1) human SH-SY5Y, 2) human Jurkat, 3) human HEL, 4) rat brain, 5) rat hippocampus, 6) mouse brain, and 7) mouse hippocampus lysates using TRIM46 Antibody / Axon Initial Segment Protein Antibody. A specific immunoreactive band is detected at approximately 83 kDa in all tested samples, consistent with the predicted molecular weight of TRIM46. These results demonstrate reliable detection of TRIM46, a neuron-associated microtubule-organizing protein that is essential for axon initial segment assembly and the establishment of neuronal polarity.