TRIM29 Antibody Mouse Monoclonal [clone TRIM29/1041] (V2507)

Description

Tripartite motif-containing protein 29 is a member of the TRIM protein family encoded by the TRIM29 gene and is also known as ATDC or ataxia-telangiectasia group D complementing protein. The TRIM29 Antibody Mouse Monoclonal TRIM29/1041 is developed to detect this epithelial-associated regulatory protein in research applications focused on DNA damage response and tumor biology. TRIM29 is located on chromosome 11q23 and, unlike many other TRIM family members, lacks a canonical RING finger domain while retaining B-box and coiled-coil motifs that support protein-protein interactions and scaffold functions.

TRIM29 has been implicated in modulation of chromatin organization, transcriptional regulation, and cellular responses to genotoxic stress. It interacts with components of the p53 signaling pathway and other regulatory proteins that influence cell cycle progression, apoptosis, and epithelial differentiation. Through these interactions, TRIM29 contributes to maintenance of genomic stability and regulation of stress-adaptive signaling pathways. Subcellular localization studies describe both cytoplasmic and nuclear distribution depending on tissue type and physiologic context.

Expression of TRIM29 is enriched in epithelial tissues including pancreas, lung, skin, and gastrointestinal tract. In cancer research, altered TRIM29 expression has been reported in pancreatic, gastric, lung, breast, and bladder carcinomas. Its biologic role appears context dependent, with evidence suggesting involvement in tumor progression or modulation of stress signaling pathways depending on tumor type. This tissue-specific behavior underscores its importance in epithelial transformation and oncogenic signaling networks.

As part of the tripartite motif protein family, TRIM29 participates in pathways regulating protein stability and cellular stress adaptation. Investigation of TRIM29 expression supports research focused on epithelial integrity, DNA damage response mechanisms, and cancer-associated signaling biology. Clone TRIM29/1041 is a mouse monoclonal antibody designed for specific detection of TRIM29 protein expression in diverse research settings.

Application Notes

Optimal dilution of the TRIM29 antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant fragment (126 amino acid residues between aa 1-200) of human TRIM29 protein was used as the immunogen for the TRIM29 antibody mouse monoclonal TRIM29/1041.

Storage

Store the TRIM29 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).