TRIM28 Antibody / KAP1 (R32210)

IHC testing of FFPE human breast cancer tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human ovarian cancer tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human gastric carcinoma tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human colorectal adenocarcinoma tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE human liver cancer tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE mouse intestinal tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat intestinal tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

Western blot testing of 1) human HeLa, 2) human PC-3, 3) human 293T, 4) human A549, 5) rat PC-12, 6) mouse spleen and 7) mouse NIH 3T3 cell lysate with TRIM28 antibody. Observed molecular weight: 88~110 kDa depending on sumoylation.

Flow cytometry testing of human U-2 OS cells with TRIM28 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM28 antibody.

Immunofluorescent staining of FFPE human ovarian cancer tissue with TRIM28 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.