TRIM16 Antibody / Tumor Suppressor Protein Antibody (RQ7582)

TRIM16 Antibody Human Colorectal Adenocarcinoma IHC. FFPE human colorectal adenocarcinoma tissue was subjected to heat-induced epitope retrieval by boiling sections in pH8 EDTA buffer for 20 minutes followed by cooling before staining with TRIM16 antibody. Strong membranous and cytoplasmic immunoreactivity is observed in the colorectal adenocarcinoma cells, with comparatively lower staining in the surrounding stromal tissue, demonstrating clear target localization and good tissue contrast. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, is suitable for studies of tumor suppressor biology, cellular differentiation, and cancer-associated signaling pathways.

TRIM16 Antibody Human Thyroid Cancer IHC. FFPE human thyroid cancer tissue was subjected to heat-induced epitope retrieval using pH8 EDTA buffer prior to staining with TRIM16 antibody at 2 ug/ml. HRP-conjugated secondary antibody with DAB chromogen was used for visualization. TRIM16 immunoreactivity is predominantly cytoplasmic with membranous accentuation in the thyroid carcinoma cells, while surrounding stromal elements exhibit comparatively weaker staining, supporting selective target detection in the neoplastic tissue. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, is suitable for studies of tumor suppressor biology, cellular differentiation, and cancer-associated signaling pathways.

TRIM16 Antibody A549 Cell IF. Immunofluorescence staining of FFPE human A549 cells was performed following heat-induced epitope retrieval by steaming tissue sections in pH6 citrate buffer for 20 minutes. TRIM16 was detected with a green fluorescent secondary antibody, while nuclei were counterstained with DAPI (blue). The staining demonstrates predominantly cytoplasmic localization with mild perinuclear enrichment, consistent with the reported intracellular distribution of TRIM16. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, is suitable for studies of tumor suppressor biology, cellular differentiation, and cancer-associated signaling pathways.

TRIM16 Antibody Human A549 Cells IF. Immunofluorescence staining of A549 cells was performed following enzyme antigen retrieval with AR0022 for 15 minutes. Cells were incubated with TRIM16 antibody at 5 ug/ml and detected using a Fluoro488-conjugated secondary antibody (green), while nuclei were counterstained with DAPI (blue). TRIM16 staining is predominantly cytoplasmic with perinuclear enrichment, consistent with the reported intracellular distribution of the protein. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, is suitable for studies of tumor suppressor biology, cellular differentiation, and cancer-associated signaling pathways.

TRIM16 Antibody Human Cancer Cell WB. Western blot analysis detected TRIM16 in human A549, HeLa, and U251 whole cell lysates. Lanes: (1) A549, (2) HeLa, and (3) U251. A distinct band was observed at approximately 64 kDa, consistent with the predicted molecular weight of TRIM16. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, demonstrates specific detection of endogenous TRIM16 and is suitable for studies of tumor suppressor biology, cellular differentiation, and cancer-associated signaling pathways.

TRIM16 Antibody A549 Cell IP. Immunoprecipitation of TRIM16 was performed from A549 whole cell lysate using TRIM16 antibody, followed by Western blot detection with the same antibody. Lanes: (1) A549 input lysate, (2) rabbit control IgG immunoprecipitation, and (3) TRIM16 antibody immunoprecipitation. A specific band at approximately 64 kDa is detected in the input and TRIM16 immunoprecipitated sample but is absent from the control IgG lane, demonstrating selective enrichment of endogenous TRIM16. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, is suitable for immunoprecipitation studies of tumor suppressor biology, protein interactions, and cancer-associated signaling pathways.

TRIM16 Antibody A549 Cell FACS. Flow cytometry analysis of fixed and permeabilized A549 cells stained intracellularly with TRIM16 antibody. The blue histogram represents cells stained with TRIM16 antibody, the green histogram represents the rabbit IgG isotype control, and the red histogram represents the unstained blank control. A clear rightward shift of the TRIM16 signal relative to both controls demonstrates specific detection of endogenous intracellular TRIM16 expression in A549 cells. This TRIM16 Antibody, also called Tumor Suppressor Protein Antibody, is suitable for flow cytometric studies of tumor suppressor biology, cellular differentiation, and cancer-associated signaling pathways.