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Home >> Antibodies >> TKT Antibody / Transketolase

TKT Antibody / Transketolase [clone 3E5.] (RQ6279)

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  Catalog No Formulation Size Price (USD)  
Image RQ6279 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human thyroid cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human thyroid cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal carcinoma with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse pancreas with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat pancreas with TKT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) HepG2, 3) SW620, 4) Raji, 5) rat liver, 6) rat RH35, 7) mouse liver and 8) mouse HEPA1-6 cell lysate with TKT antibody. Predicted molecular weight ~68 kDa.
Immunofluorescent staining of FFPE human A549 cells with TKT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with TKT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TKT antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 3E5.
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P29401
Localization Nuclear, cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Transketolase antibody is available for research use only.
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Description

Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.

Application Notes

Optimal dilution of the Transketolase antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids M1-A116) was used as the immunogen for the Transketolase antibody.

Storage

After reconstitution, the Transketolase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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