Transcriptional Corepressor BCOR Antibody / BCL6 corepressor [clone BCOR/13087R] (V5974)

Western blot analysis of Transcriptional Corepressor BCOR antibody in brain tissue lysates from multiple species. Human brain, mouse brain, rat brain, hamster brain, guinea pig brain, and rabbit brain lysates each show a prominent band at approximately 190 kDa, consistent with the predicted molecular weight of BCOR. The banding pattern is conserved across species, supporting recognition of BCOR protein in diverse mammalian brain tissues. The observed signal corresponds to full-length BCL-6 corepressor under reducing SDS-PAGE conditions, with minimal non-specific background.

Western blot analysis of Transcriptional Corepressor BCOR antibody in human brain tissue lysate. Human brain lysate shows a distinct band at approximately 190 kDa, consistent with the predicted molecular weight of BCOR. The signal appears as a single predominant band with minimal background, supporting specific detection of endogenous BCL-6 corepressor protein in neural tissue. BCOR is known to localize to the nucleus and function as a chromatin-associated transcriptional corepressor, and the observed band aligns with the expected full-length protein size under reducing SDS-PAGE conditions.

Immunohistochemistry analysis of Transcriptional Corepressor BCOR antibody in human tonsil tissue. Formalin-fixed, paraffin-embedded human tonsil stained with BCOR Recombinant Rabbit Monoclonal Antibody (clone BCOR/13087R) demonstrates predominant nuclear HRP-DAB brown staining in lymphoid cells within germinal center and interfollicular regions, consistent with the known nuclear localization of BCL-6 corepressor. Background stromal elements show minimal staining. The inset image represents the negative control in which PBS was used instead of the primary antibody, confirming absence of non-specific secondary antibody binding. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 45 minutes at 95oC followed by cooling prior to immunostaining.