- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TRAF3IP1 Antibody / TRAF3-interacting protein 1 (FY12178)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12178 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
TRAF3IP1 antibody detects TRAF3-interacting protein 1, encoded by the TRAF3IP1 gene on chromosome 2q37.3. TRAF3IP1 antibody is widely used in research on ciliogenesis, vesicle transport, and signaling pathways. TRAF3IP1, also known as IFT54, is a component of the intraflagellar transport (IFT) complex B, which mediates bidirectional transport of cargo along axonemal microtubules during cilia formation and maintenance. Its role in ciliogenesis links it to developmental processes, sensory perception, and signaling networks such as Hedgehog signaling. Expression is highest in ciliated tissues, including kidney, retina, and brain.
Structurally, TRAF3IP1 contains coiled-coil domains that mediate assembly with other IFT complex B proteins. It interacts with both microtubules and vesicular cargo, bridging the cytoskeleton with trafficking machinery. The N-terminal domain mediates binding to TRAF3, while the C-terminal region associates with kinesin motors and IFT components. This modular architecture supports its dual functions in signaling and transport.
Functionally, TRAF3IP1 plays central roles in ciliogenesis and ciliary signaling. By forming part of the IFT-B complex, it ensures delivery of structural proteins and signaling receptors to the growing cilium. Knockdown or mutation disrupts cilia formation, impairing sensory functions and developmental signaling. TRAF3IP1 also participates in TRAF3-mediated immune signaling, though this role is less characterized. Researchers use TRAF3IP1 antibody to study ciliary transport, Hedgehog signaling, and cilia-related disorders.
Clinically, mutations in TRAF3IP1 cause nephronophthisis, a ciliopathy characterized by kidney failure, retinal degeneration, and skeletal abnormalities. Additional associations include Joubert syndrome and other developmental ciliopathies. Dysregulation of TRAF3IP1 has also been observed in cancer, particularly renal carcinomas, where defective ciliogenesis contributes to tumorigenesis. NSJ Bioreagents supplies TRAF3IP1 antibody as a reliable reagent for ciliopathy research, developmental biology, and cancer studies.
Experimentally, TRAF3IP1 antibody is used in western blotting to detect the ~64 kDa protein, in immunofluorescence microscopy to study localization at basal bodies and cilia, and in immunohistochemistry to analyze expression in ciliated tissues. Immunoprecipitation with TRAF3IP1 antibody allows isolation of IFT complexes for biochemical characterization.
Optimal dilution of the TRAF3IP1 antibody should be determined by the researcher.
E.coli-derived human TRAF3IP1 recombinant protein (Position: L52-R691) was used as the immunogen for the TRAF3IP1 antibody.
After reconstitution, the TRAF3IP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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