NR2C2 Antibody / Testicular Receptor 4 Antibody (RQ8296)

NR2C2 Antibody by IP in human U2OS cells. Immunoprecipitation of NR2C2 from human U2OS whole-cell lysate using NR2C2 Antibody. Lane 1 contains 30 ug input lysate, lane 2 represents the rabbit IgG negative control, and lane 3 contains protein immunoprecipitated using 2 ug antibody and 500 ug lysate. Subsequent western blot analysis detects a prominent band at approximately 68 kDa in the input and antibody IP lanes, with no corresponding band in the IgG control. The calculated molecular weight is 65.4 kDa. This Testicular Receptor 4 Antibody demonstrates enrichment of NR2C2 under the tested conditions.

NR2C2 Antibody by WB in human, rat, and mouse samples. Western blot analysis using NR2C2 Antibody at 0.5 ug/ml. Lanes 1-4: human U2OS, HEL, Jurkat, and K562 cell lysates; lanes 5-6: rat brain and thymus; lanes 7-8: mouse brain and thymus. Each lane contained 30 ug protein separated by 10% SDS-PAGE under reducing conditions. A prominent band is detected at approximately 68 kDa, consistent with the predicted 65.4 kDa NR2C2 protein. Additional lower-molecular-weight bands are visible in several lanes, particularly the brain samples. This Testicular Receptor 4 Antibody recognizes a nuclear receptor involved in transcriptional regulation and metabolic signaling.

NR2C2 Antibody by IF in human U2OS cells. Immunofluorescence staining of human U2OS cells using NR2C2 Antibody at 5 ug/ml. NR2C2 immunoreactivity is visualized in red using a Cy3-conjugated secondary antibody, while alpha-tubulin is labeled green using a Fluoro488-conjugated secondary antibody. The red signal is concentrated predominantly within the nuclei, consistent with the expected localization of this transcriptional regulator. Green alpha-tubulin staining outlines the cytoplasmic microtubule network. Primary antibodies were incubated overnight at 4oC following enzymatic antigen retrieval. This Testicular Receptor 4 Antibody supports visualization of nuclear NR2C2 expression.

NR2C2 Antibody by IHC in mouse testis. Immunohistochemistry of FFPE mouse testis tissue using NR2C2 Antibody at 2 ug/ml. Brown DAB immunoreactivity is observed throughout the seminiferous tubules, with staining involving multiple cell populations. The signal appears predominantly cytoplasmic, with variable nuclear staining, rather than exclusively nuclear localization. Tissue sections underwent heat-induced antigen retrieval using EDTA buffer, pH 8.0, followed by overnight primary antibody incubation at 4oC. Hematoxylin counterstaining highlights the nuclei. This Testicular Receptor 4 Antibody detects immunoreactivity in a tissue associated with NR2C2-dependent reproductive regulation.

NR2C2 Antibody by WB in U2OS and Jurkat cells. Western blot analysis of human U2OS and Jurkat whole-cell lysates using NR2C2 Antibody at 0.5 ug/ml. Lane 1 contains U2OS lysate and lane 2 contains Jurkat lysate, with 30 ug protein loaded per lane and separated by 10% SDS-PAGE under reducing conditions. Both samples show a prominent NR2C2 band at approximately 68 kDa, compared with the calculated molecular weight of 65.4 kDa. An additional band near 37 kDa is visible in the Jurkat lane. GAPDH is shown as a loading control. This Testicular Receptor 4 Antibody recognizes NR2C2 in two human cell lines.