TPD52 Antibody / Tumor Protein D52 Polyclonal Antibody (FY12524)

TPD52 Antibody IHC Staining of Human Prostate Cancer Tissue. Immunohistochemical analysis of FFPE human prostate cancer tissue using Tumor Protein D52 Polyclonal Antibody at 2 ug/ml. Brown DAB staining is visible in glandular epithelial cells, with prominent cytoplasmic and peripheral cellular immunoreactivity surrounding glandular lumens. Heat-induced epitope retrieval was performed using pH 8 EDTA buffer, followed by overnight primary antibody incubation at 4°C.

TPD52 Antibody Western Blot Analysis of HepG2, HCT116, Raji and U-87 MG Cells. Western blot analysis using Tumor Protein D52 Polyclonal Antibody at 0.5 ug/ml. Lane 1: human HepG2 cells; lane 2: human HCT116 cells; lane 3: human Raji cells; lane 4: human U-87 MG cells. Immunoreactive bands are detected at approximately 25-28 kDa, near the predicted TPD52 molecular weight of 25 kDa. Multiple closely migrating bands are visible in some samples. TPD52 phosphorylation and alternative isoforms have been documented, although the individual bands have not been experimentally assigned to specific modified forms or isoforms.

TPD52 Antibody Flow Cytometry Analysis of Human HepG2 Cells. Flow cytometric analysis of fixed and permeabilized human HepG2 cells using Tumor Protein D52 Polyclonal Antibody at 1 ug per million cells. The red histogram represents unstained cells, the green histogram represents the rabbit IgG isotype control, and the blue histogram represents antibody-stained cells. The rightward shift of the blue population relative to both controls indicates detectable intracellular TPD52 immunoreactivity.

TPD52 Antibody Western Blot Validation in HCT116 Wild-Type and TPD52 Knockout Cells. Western blot analysis of human HCT116 wild-type (WT, lane 1) and TPD52 knockout (KO, lane 2) whole-cell lysates using Tumor Protein D52 Polyclonal Antibody at 0.5 ug/ml. Each lane contains 30 ug of total protein under reducing conditions. Immunoreactive bands at approximately 23-27 kDa are detected in WT lysate and are substantially reduced in the KO sample, supporting antibody specificity for TPD52. Beta-Actin serves as the loading control.