- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TP53 Antibody / Apoptosis Regulator Antibody [clone rTP53/8579] (V4440)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4440-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4440-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4440SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rTP53/8579 |
| Purity | Protein A/G affinity |
| UniProt | P04637 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT |
| Limitations | This TP53 antibody is available for research use only. |
|
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Tumor protein p53 (TP53) is a nuclear transcription factor widely recognized as one of the most important apoptosis regulators in mammalian cells. Acting as a central mediator of programmed cell death, p53 determines whether cells carrying severe genomic damage should be eliminated through apoptosis. The TP53 Antibody / Apoptosis Regulator Antibody detects this key apoptosis regulator, commonly known as p53, which activates transcriptional and mitochondrial pathways that drive programmed cell death in response to cellular stress.
TP53 antibody, also referred to as p53 antibody or Tumor protein p53 antibody in the literature, targets a protein that functions as a master apoptosis regulator within the cell. Under normal physiological conditions the apoptosis regulator p53 is maintained at very low levels through continuous ubiquitin-mediated degradation primarily controlled by the E3 ubiquitin ligase MDM2. When cells experience genotoxic stress such as DNA damage, oncogene activation, oxidative stress, or hypoxia, this regulatory pathway is disrupted, allowing stabilization and nuclear accumulation of the apoptosis regulator protein.
Once activated, the apoptosis regulator p53 functions as a sequence-specific transcription factor that induces expression of numerous pro-apoptotic genes. Among the most important transcriptional targets of the apoptosis regulator are BAX, PUMA, and NOXA, which promote mitochondrial outer membrane permeabilization and activation of the intrinsic apoptosis pathway. Through these transcriptional programs the apoptosis regulator p53 eliminates cells carrying irreparable DNA damage, preventing survival of genetically compromised cells.
In addition to its transcriptional activity, the apoptosis regulator p53 can also directly promote mitochondrial apoptosis through transcription-independent mechanisms. In this pathway p53 interacts with members of the BCL2 protein family at the mitochondrial outer membrane, facilitating release of cytochrome c and activation of caspases that execute programmed cell death. These complementary transcriptional and mitochondrial mechanisms reinforce the central role of p53 as a master apoptosis regulator in cellular stress responses.
Loss of TP53 apoptosis regulation is a common molecular event in human cancer. Mutations affecting the TP53 gene impair the ability of cells to undergo apoptosis following genomic damage, allowing survival and proliferation of genetically unstable cells. In many tumor types mutant forms of the apoptosis regulator protein become stabilized and accumulate within the nucleus, resulting in elevated p53 protein levels frequently detected in cancer tissues.
A recombinant mouse monoclonal TP53 antibody such as clone rTP53/8579 is suitable for detecting the p53 apoptosis regulator in studies examining programmed cell death pathways, tumor suppressor signaling networks, and cellular responses to genotoxic stress.
Optimal dilution of the TP53 Antibody / Apoptosis Regulator Antibody should be determined by the researcher.
Recombinant full-length human protein was used as the immunogen for the TP53 Antibody / Apoptosis Regulator Antibody.
Aliquot the TP53 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
p53 antibody, Tumor protein p53 antibody, Cellular tumor antigen p53 antibody, Phosphoprotein p53 antibody, Transformation related protein 53 antibody
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