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Home >> Antibodies >> TP53 Antibody / p53

TP53 Antibody / p53 [clone PCRP-TP53-1F7] (V8500)

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  Catalog No Formulation Size Price (USD)  
Image V8500-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V8500-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V8500SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
Microvalidated
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TP53 antibody. These results demonstrate the foremost specificity of the PCRP-TP53-1F7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human colon carcinoma with TP53 antibody (clone PCRP-TP53-1F7). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free TP53 antibody (clone PCRP-TP53-1F7) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PCRP-TP53-1F7
Purity Protein G affinity chromatography
UniProt P04637
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This TP53 antibody is available for research use only.
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Description

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Researchers studying p53 expression and TP53 signaling can explore our Protein Microarray Validated p53 Antibody page for additional reagents targeting this key tumor suppressor in cancer, DNA damage response and cell cycle research.

Application Notes

Optimal dilution of the TP53 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length TP53 protein was used as the immunogen for the TP53 antibody.

Storage

Store the TP53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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