Topoisomerase II alpha Antibody for IF / TOP2A Immunofluorescence Antibody [clone TOP2A/1361] (V3443)

Western blot testing of 1) human HepG2, 2) HeLa and 3) mouse NIH3T3 cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.

Western blot testing of human HeLa cell lysate with Topoisomerase II alpha antibody (clone TOP2A/1361). Expected molecular weight ~174 kDa.

IHC testing of FFPE human tonsil with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with Topoisomerase II alpha antibody (clone TOP2A/1361). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Topoisomerase II alpha Antibody for IF / TOP2A Immunofluorescence Antibody (clone TOP2A/1361). Immunofluorescence analysis of PFA-fixed human HeLa cells using the mouse monoclonal clone TOP2A/1361 demonstrates strong nuclear fluorescence consistent with the intracellular localization of Topoisomerase II alpha. Cells were stained with Topoisomerase II alpha antibody (green) to visualize nuclear TOP2A protein distribution, while Phalloidin (red) highlights the actin cytoskeleton and cell boundaries. The merged image shows clear nuclear immunofluorescence signal corresponding to proliferating cells, consistent with the known role of TOP2A as a DNA replication associated nuclear enzyme. The Topoisomerase II alpha Antibody for IF enables high resolution fluorescence microscopy detection of TOP2A protein and supports immunofluorescence studies examining nuclear protein localization, cell cycle progression, and proliferation associated chromatin dynamics.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Topoisomerase II alpha antibody (clone TOP2A/1361). These results demonstrate the foremost specificity of the TOP2A/1361 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Topoisomerase II alpha antibody (clone TOP2A/1361) as confirmation of integrity and purity.