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Home >> Antibodies >> TOPK Antibody / PBK

TOPK Antibody / PBK (F54731)

  Catalog No Formulation Size Price (USD)  
Image F54731-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54731-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE rat testis tissue with TOPK antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse testis tissue lysate with TOPK antibody. Predicted molecular weight ~36 kDa.
Western blot testing of human HeLa tissue lysate with TOPK antibody. Predicted molecular weight ~36 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9JJ78
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Limitations This TOPK antibody is available for research use only.
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  • Applications : WB, IHC-P, IF/ICC, FACS
    Reactivity : Human, Mouse, Rat

Description

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization (By similarity). [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the TOPK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 58-86 from the mouse protein was used as the immunogen for the TOPK antibody.

Storage

Aliquot the TOPK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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