- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TOP2A Antibody / Topoisomerase II alpha [clone TPM2A-1] (V7245)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V7245-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
|
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V7245-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
|
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V7245SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | TPM2A-1 |
| Purity | Protein G affinity chromatography |
| UniProt | P11388 |
| Gene ID | 7153 |
| Localization | Nuclear |
| Applications | Immunofluorescence : 1-2ug/ml Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml (1) |
| Limitations | This TOP2A antibody is available for research use only. |
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This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this TOP2A antibody.
Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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