- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
TOP2A Antibody / DNA Replication Enzyme Antibody [clone TPM2A-1] (V7245)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7245-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V7245-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V7245SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | TPM2A-1 |
| Purity | Protein G affinity chromatography |
| UniProt | P11388 |
| Gene ID | 7153 |
| Localization | Nuclear |
| Applications | Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml (1) |
| Limitations | This TOP2A antibody is available for research use only. |
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DNA Topoisomerase II alpha (TOP2A) is a nuclear enzyme that regulates DNA topology during DNA replication and chromatin duplication. The protein belongs to the type II topoisomerase family and functions by introducing transient double strand DNA breaks that allow one DNA helix to pass through another, relieving torsional stress generated during DNA synthesis. This activity is essential for maintaining DNA replication efficiency and genomic stability in proliferating cells.
TOP2A antibody, also known as Topoisomerase II alpha antibody or DNA Topoisomerase II alpha antibody, recognizes a nuclear enzyme widely regarded as a DNA replication enzyme because of its central role in replication fork progression and DNA synthesis. During S phase of the cell cycle, the movement of replication forks generates extensive DNA supercoiling ahead of the replication machinery. DNA Topoisomerase II alpha resolves these topological constraints, preventing replication fork stalling and allowing continued DNA strand elongation during genome duplication.
TOP2A Antibody / DNA Replication Enzyme Antibody (clone TPM2A-1) is a mouse monoclonal antibody developed to detect the nuclear enzyme responsible for relieving torsional strain generated during DNA replication. As a DNA Replication Enzyme Antibody, clone TPM2A-1 supports investigation of DNA replication dynamics, replication fork progression, and the molecular mechanisms that maintain efficient DNA synthesis during cell division. Monoclonal antibodies targeting this replication enzyme enable visualization of TOP2A protein expression in proliferating cells undergoing active DNA replication.
The designation DNA Replication Enzyme Antibody differentiates this TOP2A antibody page from other TOP2A antibody pages that emphasize alternative biological roles of the protein. While some TOP2A antibodies are described as Topoisomerase II alpha antibodies, chromosome segregation enzyme antibodies, or cell proliferation marker antibodies, this antibody page specifically highlights the enzyme's role in replication fork progression and DNA topology control during DNA replication. Structuring antibody pages around these biological roles is a powerful differentiation strategy used by antibody vendors to ensure multiple antibodies targeting the same protein are interpreted by search engines as distinct research reagents.
DNA Topoisomerase II alpha activity increases dramatically in cells entering S phase and remains elevated during periods of active DNA synthesis. Because the enzyme resolves DNA supercoiling generated by rapidly moving replication forks, TOP2A is essential for successful genome duplication. Antibodies recognizing this DNA replication enzyme therefore support research investigating replication stress responses, DNA synthesis regulation, and mechanisms that preserve genome integrity during cell proliferation.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A Antibody / DNA Replication Enzyme Antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this TOP2A Antibody / DNA Replication Enzyme Antibody.
Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Topoisomerase II alpha antibody, DNA Topoisomerase II alpha antibody, TOP2 antibody, Topoisomerase IIa antibody, DNA decatenation enzyme antibody
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