TOP2A Antibody / Chromosome Segregation Enzyme Antibody [clone TOP2A/1362] (V3442)

TOP2A Antibody / Chromosome Segregation Enzyme Antibody (clone TOP2A/1362). Immunohistochemistry analysis of FFPE human tonsil tissue demonstrates strong nuclear staining consistent with expression of the chromosome segregation enzyme DNA Topoisomerase II alpha in proliferating lymphoid cells. Nuclear immunoreactivity is concentrated within germinal center lymphocytes, reflecting the high mitotic activity of these cells during immune responses. The Chromosome Segregation Enzyme Antibody highlights proliferating germinal center B cells where mitotic chromosome segregation and chromatid decatenation are actively occurring, while surrounding mantle zone lymphocytes show minimal staining. Required HIER: boil tissue sections in pH 9 Tris-EDTA buffer for 10-20 min followed by cooling at RT for 20 min prior to immunohistochemistry staining.

IHC testing of FFPE human bladder carcinoma with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

TOP2A Antibody / Chromosome Segregation Enzyme Antibody (clone TOP2A/1362). Immunofluorescence analysis of PFA-fixed human HeLa cells demonstrates strong nuclear staining of DNA Topoisomerase II alpha, a chromosome segregation enzyme that functions during mitosis to resolve DNA catenanes and enable sister chromatid separation. Green fluorescence highlights nuclear localization of the TOP2A protein in proliferating cells, consistent with its role in chromosome decatenation and mitotic chromosome segregation. Phalloidin staining (red) marks the actin cytoskeleton, outlining cell morphology and providing cytoplasmic reference while TOP2A signal remains predominantly nuclear. The Chromosome Segregation Enzyme Antibody enables visualization of the nuclear enzyme responsible for chromatid decatenation and proper chromosome partitioning during cell division in immunofluorescence assays.

TOP2A Antibody / Chromosome Segregation Enzyme Antibody (clone TOP2A/1362). Western blot analysis of human HeLa cell lysate following SDS-PAGE separation and membrane transfer demonstrates detection of DNA Topoisomerase II alpha. A band is detected at approximately 170-180 kDa, consistent with the predicted molecular weight of DNA Topoisomerase II alpha / TOP2A (~174 kDa). The signal corresponds to the chromosome segregation enzyme responsible for resolving DNA catenanes and enabling sister chromatid separation during mitosis. Detection of the denatured TOP2A protein band confirms the utility of this Chromosome Segregation Enzyme Antibody for western blot analysis of this essential mitotic enzyme in human cell lysates.

TOP2A Antibody / Chromosome Segregation Enzyme Antibody (clone TOP2A/1362). HuProt human protein microarray specificity analysis using an array containing more than 19,000 full-length human proteins demonstrates highly selective recognition of DNA Topoisomerase II alpha by clone TOP2A/1362. The Chromosome Segregation Enzyme Antibody shows a dominant signal for the intended target TOP2A with a Z-score of 134.27 and S-score of 124.98, indicating exceptionally strong binding to DNA Topoisomerase II alpha relative to other proteins present on the array. These results confirm that this monoclonal antibody preferentially recognizes the chromosome segregation enzyme responsible for DNA decatenation and mitotic chromatid separation.
Z- and S-score explanation: The Z-score represents the strength of signal generated when the antibody, together with a fluorescently labeled secondary antibody, binds to a specific protein on the HuProt microarray. Z-scores are expressed as the number of standard deviations above the mean signal intensity across the entire protein array. When proteins on the array are ranked by decreasing Z-score, the S-score represents the difference between the Z-scores of neighboring ranked proteins. The S-score therefore reflects the relative specificity of an antibody for its intended target protein compared with other proteins present on the microarray.

SDS-PAGE analysis of purified, BSA-free TOP2A antibody (clone TOP2A/1362) as confirmation of integrity and purity.