TOP2A Antibody / Topoisomerase II alpha [clone TOP2A/1362] (V3442)

IHC testing of FFPE human tonsil with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human HeLa cells with TOP2A antibody (green, clone TOP2A/1362) and Phalloidin (red).

Western blot testing of human HeLa cell lysate with TOP2A antibody (clone TOP2A/1362). Expected molecular weight ~174 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TOP2A antibody (clone TOP2A/1362). These results demonstrate the foremost specificity of the TOP2A/1362 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free TOP2A antibody (clone TOP2A/1362) as confirmation of integrity and purity.