TOP2A Antibody for IF / Topoisomerase II Alpha Immunofluorescence Antibody (RQ5605)

TOP2A Antibody for IF / Topoisomerase II Alpha Immunofluorescence Antibody. Immunofluorescence analysis of FFPE human U-2 OS cells using a TOP2A antibody reveals distinct nuclear fluorescence consistent with the known localization of Topoisomerase II alpha in proliferating cells. Cells were stained with TOP2A antibody (green) to visualize nuclear distribution of the DNA topoisomerase II alpha protein, while DAPI (blue) highlights cell nuclei. Antigen retrieval was performed by steaming sections in pH6 citrate buffer prior to staining to enable optimal immunofluorescence detection in FFPE cells. The merged immunofluorescence pattern demonstrates nuclear enrichment of TOP2A signal within U-2 OS cells, consistent with the role of this enzyme in DNA replication and chromosome segregation. This IF staining pattern supports the use of the TOP2A Antibody for IF in immunofluorescence microscopy studies examining nuclear protein localization, proliferating cell populations, and cell cycle associated chromatin dynamics.

IHC staining of FFPE human lung cancer with TOP2A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human rectal cancer with TOP2A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Western blot testing of human 1) A549, 2) U-2 OS, 3) HEK293 and 4) K562 cell lysate with TOP2A antibody. Expected molecular weight ~174 kDa.

Flow cytometry testing of human 293T cells with TOP2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TOP2A antibody.