TOP2A Antibody for IF / Topoisomerase II Alpha Immunofluorescence Antibody (RQ5605)

TOP2A Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells using TOP2A Antibody for IF / Topoisomerase II Alpha Immunofluorescence Antibody. Heat-induced epitope retrieval was performed using pH6 citrate buffer prior to staining. TOP2A immunoreactivity (green) is predominantly localized to the nuclei, while DAPI (blue) counterstains cell nuclei. The staining pattern is consistent with the expected nuclear localization of Topoisomerase II alpha in proliferating cells. This TOP2A Antibody for IF is suitable for studies of nuclear protein localization, DNA replication, chromosome segregation, and cell cycle regulation.

TOP2A Antibody U-2 OS Nuclear IF. Immunofluorescent staining of fixed and permeabilized human U-2 OS cells using TOP2A Antibody for IF / Topoisomerase II Alpha Immunofluorescence Antibody at 5 ug/ml. Cells underwent enzyme-mediated antigen retrieval for 15 minutes, followed by detection with a Fluoro488-conjugated goat anti-rabbit IgG secondary antibody (green). TOP2A staining is predominantly localized to the nuclei, consistent with the expected distribution of this DNA topoisomerase in proliferating cells. This TOP2A Antibody for IF is suitable for studies of nuclear protein localization, DNA replication, chromosome segregation, and cell cycle regulation.

TOP2A Antibody Human Rectal Cancer IHC. Immunohistochemical staining of FFPE human rectal cancer tissue using TOP2A Antibody / Topoisomerase II Alpha Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH 8.0 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Predominantly nuclear staining is observed in the tumor cells, consistent with the expected localization of TOP2A in actively proliferating cells. This TOP2A Antibody, also called Topoisomerase II Alpha Antibody, is suitable for studies of cell proliferation, DNA replication, chromosome segregation, and cancer biology.

TOP2A Antibody Human, Rat and Mouse WB. Western blot analysis of human HaCaT (lane 1), A549 (lane 2), K562 (lane 3), and U-2 OS (lane 4) whole cell lysates, along with rat C6 (lane 5) and mouse Neuro-2a (lane 6) whole cell lysates using TOP2A Antibody / Topoisomerase II Alpha Antibody at 0.5 ug/ml. A specific band was detected at approximately 174 kDa in all samples, consistent with the expected molecular weight of TOP2A. This TOP2A Antibody, also called Topoisomerase II Alpha Antibody, is suitable for studies of DNA replication, chromosome segregation, cell proliferation, and cancer biology.

TOP2A Antibody HepG2 Cell FACS. Flow cytometric analysis of fixed and permeabilized human HepG2 cells using TOP2A Antibody / Topoisomerase II Alpha Antibody at 1 ug/10^6 cells. The blue histogram represents TOP2A staining, the green histogram shows the rabbit IgG isotype control, and the red histogram represents the unstained control. A clear rightward shift of the TOP2A-stained population demonstrates specific intracellular detection of TOP2A. This TOP2A Antibody, also called Topoisomerase II Alpha Antibody, is suitable for studies of cell proliferation, DNA replication, chromosome segregation, and cancer biology.