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Home >> Antibodies >> TOP1MT Antibody

TOP1MT Antibody [clone TPIMT-1] (V7046)

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  Catalog No Formulation Size Price (USD)  
Image V7046-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7046-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7046SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7046IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Western blot testing of human Jurkat cell lysate with TOP1MT antibody (clone TPIMT-1). Predicted molecular weight ~66 kDa.
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Western blot testing of human Jurkat cell lysate with TOP1MT antibody (clone TPIMT-1). Predicted molecular weight ~66 kDa.
IHC testing of FFPE human melanoma with TOP1MT antibody (clone TPIMT-1). Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human pancreas with TOP1MT antibody (clone TPIMT-1). Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name TPIMT-1
Purity Protein G affinity chromatography
UniProt Q969P6
Localization Cytoplasmic (mitochondria)
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This TOP1MT antibody is available for research use only.
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Description

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Application Notes

Optimal dilution of the TOP1MT antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.

Storage

Store the TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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