Toll-Interacting Protein Antibody / TOLLIP Polyclonal Antibody (R31112)

Toll-Interacting Protein Antibody IHC Staining of Human Ovarian Cancer Tissue. Immunohistochemical analysis of FFPE human ovarian cancer tissue using TOLLIP Polyclonal Antibody. Brown immunoreactivity is observed in tumor-associated cells, with predominantly cytoplasmic staining and heterogeneous distribution throughout the tissue section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

Toll-Interacting Protein Antibody IHC Staining of Human Lung Cancer Tissue. Immunohistochemical analysis of FFPE human lung cancer tissue using TOLLIP Polyclonal Antibody. Brown immunoreactivity is distributed among tumor-associated cells, with prominent cytoplasmic staining and variation in signal intensity across the tissue section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

Toll-Interacting Protein Antibody IHC Staining of Mouse Brain Tissue. Immunohistochemical analysis of FFPE mouse brain tissue using TOLLIP Polyclonal Antibody. Brown immunoreactivity is observed in scattered cells and cellular processes within the brain tissue, with heterogeneous staining against the surrounding neuropil. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

Toll-Interacting Protein Antibody IHC Staining of Rat Brain Tissue. Immunohistochemical analysis of FFPE rat brain tissue using TOLLIP Polyclonal Antibody. Brown immunoreactivity is visible in neuronal cell bodies and surrounding tissue, with predominantly cytoplasmic staining and variation among individual cells. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

Toll-Interacting Protein Antibody IF Staining of Human U-2 OS Cells. Immunofluorescence analysis of FFPE human U-2 OS cells using TOLLIP Polyclonal Antibody. Red TOLLIP immunoreactivity is distributed throughout the cytoplasm, with areas of concentrated perinuclear staining surrounding blue DAPI-stained nuclei. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes.

Toll-Interacting Protein Antibody WB Analysis of Human RT4, U-2 OS, SW620 and HeLa Cells, Rat Brain and Mouse Brain. Western blot analysis of human RT4 (lane 1), U-2 OS (lane 2), SW620 (lane 3), and HeLa (lane 4) cell lysates, rat brain tissue lysate (lane 5), and mouse brain tissue lysate (lane 6) using TOLLIP Polyclonal Antibody. Immunoreactive bands are detected near 30 kDa across all six samples, consistent with the predicted molecular weight of TOLLIP.

Toll-Interacting Protein Antibody FACS Analysis of Human Daudi Cells. Flow cytometry analysis of human Daudi cells using TOLLIP Polyclonal Antibody at 1 ug per million cells following blocking with goat serum. The blue histogram represents antibody-stained cells, the green histogram represents the isotype control, and the red histogram represents unstained cells. The antibody-stained population exhibits a rightward fluorescence shift relative to both controls.

Toll-Interacting Protein Antibody IP Analysis of Human HeLa Cell Lysate. Immunoprecipitation of TOLLIP from 500 ug of human HeLa whole-cell lysate using 2 ug of TOLLIP Polyclonal Antibody. The immunoprecipitated material was analyzed by Western blot, with an immunoreactive band detected near 30 kDa, consistent with the expected molecular weight of TOLLIP. The experiment demonstrates recovery of TOLLIP from HeLa cell lysate under the tested immunoprecipitation conditions.