- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Tollip Antibody / Toll-Interacting Protein (R31112)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R31112 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q9H0E2 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells Immunoprecipitation : 2ug per 500ug of lysate |
| Limitations | This Tollip antibody is available for research use only. |
|
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Tollip antibody targets Toll-interacting protein (Tollip), a cytoplasmic adaptor protein that functions as a negative regulator of innate immune signaling. Tollip plays a key role in modulating signaling downstream of Toll-like receptors and the interleukin-1 receptor by interacting with receptor complexes and associated kinases. Through these interactions, Tollip helps fine-tune inflammatory responses and prevents excessive activation of immune signaling pathways. The protein localizes primarily to the cytoplasm and endosomal compartments, consistent with its involvement in receptor-proximal signaling and trafficking events.
Functionally, Tollip acts as an inhibitory adaptor that dampens activation of NF-kappa B and related inflammatory pathways following receptor stimulation. It can associate with ubiquitinated signaling components and influence their stability or localization, thereby regulating signal duration and intensity. Tollip expression is broadly observed across immune and non-immune cell types, reflecting its general role in maintaining immune homeostasis. A Tollip antibody supports studies examining regulation of innate immune signaling and inflammatory control mechanisms.
Tollip also participates in endosomal trafficking and autophagy-related processes, linking immune receptor signaling with intracellular protein handling pathways. By interacting with phosphoinositides and endosomal membranes, Tollip helps coordinate receptor sorting and signal attenuation following ligand engagement. Disruption of Tollip function can lead to heightened inflammatory signaling and altered cellular responses to microbial products or tissue damage. A Tollip antibody enables investigation of Tollip expression and subcellular distribution in immune and signaling studies.
From a biological and disease-relevance perspective, Tollip has been studied in the context of inflammatory disease, infection, and tissue injury, where dysregulated innate immune signaling contributes to pathology. Altered Tollip expression or function has been associated with increased susceptibility to inflammatory disorders and impaired resolution of immune responses. Understanding Tollip regulation provides insight into mechanisms that balance host defense with prevention of chronic inflammation.
At the molecular level, Tollip is encoded by the TOLLIP gene and produces a protein of approximately 274 amino acids. The protein contains a C2 domain involved in membrane association and regions that mediate protein-protein interactions with signaling adaptors and ubiquitinated substrates. Regulation of Tollip activity is influenced by cellular context, receptor engagement, and inflammatory cues. A Tollip antibody supports research applications focused on innate immunity, receptor signaling, and inflammatory regulation, with NSJ Bioreagents providing reagents intended for research use.
The stated application concentrations are suggested starting amounts. Titration of the Tollip antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of human Toll-Interacting Protein (NAQPRCSEEDLKAIQDMFPN) was used as the immunogen for this Tollip antibody.
After reconstitution, the Tollip antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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