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Home >> Antibodies >> Toll-like Receptor 1 Antibody / TLR1

Toll-like Receptor 1 Antibody / TLR1 (RQ6074)

  Catalog No Formulation Size Price (USD)  
Image RQ6074 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human ThP-1 cells with Toll-like Receptor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Toll-like Receptor 1 antibody.
Flow cytometry testing of human A549 cells with Toll-like Receptor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Toll-like Receptor 1 antibody.
Western blot testing of human 1) ThP-1 and 2) U-87 MG cell lysate with Toll-like Receptor 1 antibody. Predicted molecular weight ~90 kDa.
IHC staining of FFPE rat spleen with Toll-like Receptor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q15399
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Toll-like Receptor 1 antibody is available for research use only.
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Description

Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.

Application Notes

Optimal dilution of the Toll-like Receptor 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids T62-D404) was used as the immunogen for the Toll-like Receptor 1 antibody.

Storage

After reconstitution, the Toll-like Receptor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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