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Home >> Antibodies >> TNR Antibody / Tenascin-R

TNR Antibody / Tenascin-R (R30781)

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  Catalog No Formulation Size Price (USD)  
Image R30781 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse brain tissue with TNR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain tissue with TNR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with TNR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioma tissue with TNR antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing 1) mouse brain and 2) rat brain tissue lysate with TNR antibody. Predicted molecular weight ~150 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with TNR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TNR antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q92752
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This TNR antibody is available for research use only.
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Description

Tenascin-R is a protein that in humans is encoded by the TNR gene. It is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (Hexabrachion), TNX (TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that Tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the TNR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the N-terminus of mouse Tenascin-R (QTSDHESQVTFTHK) was used as the immunogen for this TNR antibody (100% rat homology).

Storage

After reconstitution, the TNR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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